What is Phosphate Buffered Saline with Tween-20 and its main uses?

Phosphate Buffered Saline with Tween-20 (PBST) is a versatile buffer solution commonly used in biological and biochemical applications. Its main uses include diluting samples, washing steps in various assays, and serving as a medium for cell culture. The presence of Tween-20 acts as a surfactant, enhancing the solubility of proteins and other biomolecules, which is crucial for applications like ELISA or Western blotting. Researchers often rely on PBST for its stability and compatibility with various macromolecules.

What does the 0.1um filtration process ensure?

The 0.1um filtration process ensures that the PBST buffer is sterile and free from contaminants, such as bacteria and fungi, which can interfere with experimental results. This level of filtration is significant in biological experiments to maintain the integrity of cell cultures and assays. It allows scientists to perform sensitive procedures without introducing unwanted variables from microbial contaminants, making it ideal for both laboratory and clinical research.

Why is the pH level of 7.4 important in PBST?

A pH level of 7.4 is important in PBST because it closely matches the physiological pH of human blood, which is crucial for many biological assays and experiments. Maintaining this pH is essential for protein stability and function, ensuring accurate results in immunological and biochemical assays. For instance, when performing immunoassays, using PBST at pH 7.4 helps to preserve antibody binding affinity and specificity, thus enhancing assay reliability.

Is this PBST buffer ready to use directly out of the bottle?

Yes, this PBST buffer is ready to use directly out of the bottle, which simplifies preparation and ensures consistency in your experiments. Being ready to use eliminates the hassle of diluting or adjusting components before use, allowing researchers to save valuable time. This feature is particularly beneficial in high-throughput laboratories where efficiency is key, enabling researchers to focus on their experiments rather than buffer preparation.

What types of applications can benefit from using PBST?

PBST is beneficial for a wide range of applications, including enzyme assays, protein western blotting, and immunostaining procedures. Its buffering capacity and surfactant properties make it ideal for washing steps in assays, ensuring that unbound substances are removed without affecting the bound analytes. Additionally, PBST is useful in cell culture applications where maintaining sterility and physiological conditions are crucial for cell viability.

How should PBST be stored to maintain its quality?

PBST should be stored at 2-8°C in a tightly sealed container to maintain its quality and effectiveness. Proper storage prevents degradation and contamination of the buffer, which could compromise experimental results. It's important to avoid repeated freeze-thaw cycles, as this can alter the pH and the integrity of the surfactant. Following these storage guidelines ensures that you have a reliable reagent ready for your next experiment.

Can PBST be used for cell culture applications?

Yes, PBST can be used in certain cell culture applications, particularly where washing of cells is required. Its sterile nature and physiological pH make it suitable for rinsing cells or diluting protective agents during culture assays. For researchers working with sensitive cell lines, using PBST helps maintain cell health and ensure experimental accuracy. The addition of Tween-20 also aids in reducing surface tension, promoting better cell detachment when needed.

What is the significance of Tween-20 in this buffer?

Tween-20 is a non-ionic surfactant that enhances the solubility of proteins and other biomolecules in the PBST buffer. Its significance lies in its ability to reduce nonspecific binding during assays, which is essential for obtaining clear and accurate results. When performing procedures like ELISAs or Western blots, using PBST with Tween-20 can improve the signal-to-noise ratio, allowing for more reliable detection of target molecules and minimizing background interference.

Is this buffer suitable for use in immunoassays?

Absolutely, this PBST buffer is highly suitable for use in immunoassays due to its effective property of reducing nonspecific binding. The combination of phosphate buffer and Tween-20 allows for the optimal performance of both antibodies and antigens, ensuring reliable interactions during testing. Many laboratories utilize this buffer for enzyme-linked immunosorbent assays (ELISAs) and Western blotting, where precise binding conditions are crucial for obtaining valid and repeatable results.

Where can I buy Phosphate Buffered Saline with Tween-20 in Laos?

You can purchase Phosphate Buffered Saline with Tween-20 from Ubuy in Laos. Ubuy offers a convenient online shopping experience where you can find a range of laboratory reagents, including high-quality PBST buffers. This ensures that you receive a reliable product, suitable for various scientific applications, directly to your doorstep.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

CryoKing

Phosphate Buffered Saline with Tween-20, PBST Buffer 1X, 500mL

Our PBST solution is pre-prepared and ready to go right out of the bottle—no need for preparation, dilution, or additional handling.

CryoKing

Item #: 221926695

Phosphate Buffered Saline with Tween-20, PBST Buffer 1X, 500mL

90% of respondents would recommend this to a friend

Item #: 221926695

LAK 560867

Price Details

Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )

^{*All items will import from US}

Availability : In stock

Imported from USA store

QTY:

Order now and get it around Friday, October 16

Secured transaction

Our Top Logistics Partners

Our PBST solution is pre-prepared and ready to go right out of the bottle—no need for preparation, dilution, or additional handling.

U-Care Warranty:

None

Select a Plan

Fast
Shipping

Free
Return^*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

Note: Step Down Voltage Transformer required for using electronics products of US store (110-120). Recommended power converters Buy Now.

What Stands Out

Ready To Use

Eliminates preparation time, offering immediate usability for laboratory applications, ensuring convenience for researchers and saving valuable time during experiments.

Sterile Filtration

Guaranteed 0.1um filtration sterilization that minimizes contamination risks, providing confidence and reliability in sensitive biological experiments.

Optimal pH Balance

Maintains a stable pH of 7.4, critical for biological stability, ensuring consistent conditions for various biochemical assays and processes.

Product Details

Country of Origin	This item will be imported from US
Item Weight	1.1 lbs (500 grams)
What is in the box	Phosphate Buffered Saline with... For more details, please check description/product details

Who Should Buy?

Biological Researchers
This buffer is ideal for researchers conducting cellular and molecular biology experiments requiring a stable pH environment.
Diagnostic Laboratories
Perfect for labs performing diagnostic assays that require a non-toxic, sterile environment for accurate results.
Cell Culture Applications
Suitable for use in cell culture systems to maintain osmotic balance and support cell viability.

High-Volume Productions
Not ideal for large-scale productions requiring bulk solutions, as this comes in a smaller 500ml bottle.

Product Description

Phosphate Buffered Saline with Tween-20, PBST Buffer 1X, 500mL

Have any Query? Chat with us

Customer Questions & Answers

Question: How to Shop Phosphate Buffered Saline with Tween-20, PBST Buffer Online From Ubuy?

Answer: It’s easy to shop Phosphate Buffered Saline with Tween-20, PBST Buffer online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Phosphate Buffered Saline with Tween-20, PBST Buffer Available to Shop Online in Laos?

Answer: Yes, at Ubuy Laos this product is available for you to shop at a reasonable price. The Phosphate Buffered Saline with Tween-20, PBST Buffer is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

CryoKing Buffers Editorial Review

Phosphate Buffered Saline with Tween-20, PBST Buffer 1X, 500mL is a ready-to-use laboratory solution manufactured by BIOLOGIX under the CryoKing brand. This buffer features 0.05% Tween-20 and has a pH of 7.4, ensuring optimal conditions for various biochemical applications. The solution has been sterilized through 0.22μm filtration, providing a reliable buffer that meets laboratory standards. Users appreciate its convenience for immediate use, which makes it suitable for experiments requiring PBST without additional preparation. Overall, the quality and readiness of this product help save time in essential laboratory settings.

Customer Reviews & Ratings

4.0

1 customers ratings

5 Star

0%
4 Star

100%
3 Star

0%
2 Star

0%
1 Star

0%

Review this product

Share your thoughts with other customers

Pros

Ready-to-use solution saves preparation time
Sterilized for reliable laboratory use
Maintains optimal pH for various experiments
Filter sterilized for safety
Lightweight and easy to handle

Cons

Limited availability in larger sizes

Product Price History

Important information

Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
Not all the products listed on Ubuy are for sale, as Ubuy is a global search engine. Products are subject to export/trade regulations.

LAK 560867

Order now and get it around Friday, October 16

QTY:

Secured transaction

PCI DSS compliant and ISO 27001:2022 certified, with encrypted payments and full buyer protection on every order.

Our Top Logistics Partners

Fastest cross-border delivery

Features & Benefits

Reliable formula with ideal pH buffering for consistent lab results.
Enhanced performance with Tween-20 for effective washing steps.
Low endotoxin levels due to one-step injection molding.
Ready-to-use solution that eliminates preparation time.
Long shelf life of 3 years when stored at room temperature.
Streamlines laboratory workflows for ongoing research.

Ubuy Assurance

Experience worry-free shopping with 100% original products, PCI DSS-compliant payment security, ISO 27001-certified data protection, the fastest cross-border delivery, free returns ^*, and secure packaging on every order.

Phosphate Buffered Saline with Tween-20, PBST Buffer 1X, 500mL

LAK 560867

Fast Shipping

Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Product Details

Who Should Buy?

Product Description

Customer Questions & Answers

CryoKing Buffers Editorial Review

Customer Reviews & Ratings

Review this product

Pros

Cons

Product Price History

Important information

LAK 560867

Features & Benefits

Ubuy Assurance

Fast
Shipping

Free
Return^*